Cu-Free Click Reagents – A Guide to a Few Important Azides and Alkynes

For antibody labeling and for several chemical reaction processes, Cu-Free Click Reagents are getting used these days. Reagents have big roles to play in modern day system. They do not only help in major findings but also help the scientists to understand many new aspects of thermo chemistry, modern chemistry and various other domains of chemistry. When it comes to reagents, various kinds of them are there and they are naturally very expensive. To buy them, one has to make sure finding a trusted source so that authentic reagents can be bought. In this article, we shall be introduced to a few Cy-reagents. Have a look on the following portion of the article.

1. Cy3 – Azide

At top of the list, we have Cy3 – Azide, which is an Azide activated florescent dye that has been often used for chemoselective labeling. In salient chemical reactions, it has been used to abolish the akyne tagged molecules. This reagent has been characterized as well as recognized by its high soluble ability in water. It gets dissolved in water miscible solvents quite easily. The azide group of Cy3 – azide reacts with the terminal alkynes and due to this reaction stable trizole has been produced. This reaction is a matter of biocompatibility and the reaction takes place in the normal temperature.

2. Cy5-Alkyne

For chemo selective labeling, Cy5-Alkyne has been used widely and effectively. It has been mainly used for detection of the azido tagged molecules. With the help of florescent imaging and metabolic labeling, it can ascertain the azido tagged molecules. One of the leading characteristic of this reactor is that it is highly soluble in water miscible substances or solvents. It has to be noted that the azide and alkyne do not react with each other, though they can get conjugated to each other with high efficiency. The terminal alkyne group can react with the terminal azide group and then a stable tiazole has been formed. This reaction process is a matter of biocompatibility and selectivity. This reagent has power to form covalent bonds in living organisms.

3. Cy5 – Azide

This is another popular reactor or reagent that can be found in the modern chemistry or biochemistry laboratories in these days. For detection of alkyne tagged molecules and for chemoselective labeling, this azide activated florescent dye has been widely used. This reactor has been recognized by its supreme character of getting dissolved water miscible substances. The azide group of this reactor can react with terminal alkynes and as the result of the reaction stable triazole has been produced.

About the Author

Cy5 – Azide this is another popular reactor or reagent that can be found in the modern chemistry or biochemistry laboratories in these days. For more details click at clickchemistrytools.com.

DBCO-based building blocks, labeling reagents, haptens, and fluorescent probes for Cyclooctynes, Cu-free click chemistry. Click Chemistry Tools offers small molecule conjugation, protein-protein conjugation and modification of proteins with bioorthog