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Fluorescent Azide – A Gateway to Click Chemistry

Author: Leonardo Dicaprio
by Leonardo Dicaprio
Posted: Jan 13, 2015

Those, who are health conscious or even love science, often prefer to read newspapers or magazines to get the major health related reports and inventions. It has to be stated that modern science has progressed a lot and the progress is even beyond our imagination. Thanks to the technology, we have come to know that cancer happens due to genetic reasons. Reading the structures of genes properly can crack many health related mysteries. From this, life saving medicines has been produced to counter complicated genetic diseases. Modern bio-technology or bio-chemistry is all about experimenting different structures of genes or DNA. For carrying out experiments with genes or genetic structures, reagents are required, which are used to make DNA structures simplified to be understood.

A Guide to Click Chemistry

Click chemistry is a part of modern day bio-chemistry. In click chemistry, different reagents are studied and their properties, as well as applications are understood. It is basically defined as an approach for easy, effective, accurate and simplified labeling as well as detection of the bio-molecules. For example, DBCO-TAMRA is a reagent that has been used for sample labeling, conjugation and derivation process. With copper catalyzed medium, an alkyne and an azide undergo chemical reactions to form stable triazole. This is was notably the first step towards Click Chemistry, which was concluded in the year of 1970s, by famous chemistry expert Rolf Huisgen. The addition of Copper catalyst makes this reaction possible in room temperature.

Bio-Orthogonal Labeling with Fluorescent Chromeo Dyes

Fluorescent Azide dye has huge applications in the field of click chemistry. Several Chromeo dyes are there as well, which have superior fluorescent properties in offering. The term click chemistry is basically used for describing a group of few effective as well as selective reactions that are often used for reading complicated DNA structures. Bio labeling is a process, where fluorescent dyes play major roles, especially Fluorescent DBCO groups of reagents have major roles to play.

How to Get the Reagents

These reagents are available in standard forms at chemist dealers. Reagents, like Fluorescent Tetrazines, are found to be very costly and they are available in specific quantity for the buyers. Individual researchers or laboratories can buy these reagents if they hold certificates. If you are looking for Desthiobiotin-Azide or other reagents, then the best place to buy them is probably the online stores. You can place order through online stores conveniently and some of the stores offer detailed general information on these reagents.

About the Author

Fluorescent Azide dye has huge applications in the field of click chemistry. For getting more about Fluorescent Azide visit at

About the Author

DBCO-based building blocks, labeling reagents, haptens, and fluorescent probes for Cyclooctynes, Cu-free click chemistry. Click Chemistry Tools offers small molecule conjugation, protein-protein conjugation and modification of proteins with bioorthog

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  • hannahroberts  -  3 years ago

    Click chemistry with metabolic labeling has been widely used for selectively imaging biomacromolecules in cells. The first example of azide-alkyne cycloaddition for ratiometric fluorescent imaging of live cells is reported. Alfa Chemistry-Chemistry Supplier

Author: Leonardo Dicaprio

Leonardo Dicaprio

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United Kingdom

Member since: Dec 10, 2014
Published articles: 8

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