Caffeine and the Caffeine ELISA Kit
As familiarized by all scientific staff, the
ELISA is a test usually used to identify a
substance by using antibodies and color change
.The Caffeine ELISA Kit kit is for the
quantitative and/or qualitative screening of
Caffeine in water, urine, and saliva samples.
To understand the Caffeine ELISA Kit, there
is a need to talk about the Caffeine.
1. Is the Caffeine common?
Actually, we are familiar with Caffeine, for
it can be easily found in our life. It is said
that it is discovered in over sixty plants,
such as coffee beans, tea leaves, etc.
Therefore, you cannot say you never get in
touch with it. you might drink coffee to get
rid of tiredness and weariness, ; you taste a
cup of green tea in the pleasant Sunday
afternoon, or enjoy a bar of chocolate in
leisure time, drain a bottle of soda or energy
drinks after running, and jelly beans, gums
for the young people, etc.; almost all of us
use caffeine in one form or another in our
life.
Not only in daily life, Caffeine is also used
in medical field. Usually, it is added to
certain medications, like pain killers, aiming
to strengthen the medicine effectiveness. It
can also be used for the treatment of Migraine and headaches or breathing problems of babies.
2. What the effect of Caffeine is?
According to The Food and Drug Administration
(FDA) in US, caffeine is both a drug and a
food additive. Although it is the most
commonly used drug in the world, excessive
caffeine intake can also cause health problems
and side effects on our bodies despite of the
benefits it brings in our daily life.
As a matter of fact, Caffeine is a central
nervous system stimulant, and a excessive
caffeine consumption of can lead to relatively
long term complications, whether it is
consumed in food or as a medicine. Take the
coffee as an example, if you drink more than
500 to 600 mg (around 6 cups of brewed coffee)
a day may cause side effects on your body.
You may have symptoms such as nervousness,
concentrating difficulty, muscle tremors, high
blood pressure, fast heart beat, stomach
upset and irritability, insomnia, dependency,
etc.
Once consumed, an effect that may begin as
early as 15 minutes and can last for as long a
s six hours. You can imagine how crazy you are
when you cannot sleep every night.
To prevent side effects it takes, you need be
careful in life. If you are seeing doctors for
being depression, anxiety or insomnia, heart
problems, high blood pressure or kidney
disease, you should avoid the excessive
caffeine consumption. If you are pregnant, or
planning to become pregnant, you need
eliminate caffeine or limit intake to
200-300 mg per day.
3. How the Caffeine ELISA Kit test?
After the introducing of Caffeine, this part
is about the biological test. It is a direct
competitive ELISA which is based on the
recognition of Caffeine by specific antibodies
. Caffeine, when present in a sample, and a
Caffeine
- HRP analogue compete for the binding sites
of mouse anti-Caffeine antibodies in
solution.The Caffeine antibodies are then
bound by a second antibody
(goat anti-mouse) immobilized on the wells of
the microtiter plate.
After a washing step and addition of the
substrate solution, a color signal is
generated. The intensity of the blue color
is inversely proportional to the concentration
of Caffeine present in the sample. The color
reaction is stopped after a specified time
and the color is evaluated using an ELISA
reader. The concentrations of the samples are