Nanobody — A Up-rising Star in Antibody Research
In today’s market of pharmaceutical industry, the development of therapeutic antibody drugs, known as biological missile, is extremely active, but their common characteristics hinder their way to clinical application and promotion in the market. They are oversized, expensive and too sophisticated in structure. Nanobody, however, can greatly overcome these shortcomings. It is smaller in size, better in effect as well as cheaper in price.
What is Nanobody?
Nanobody?also called domain antibody or sdAb, is an antibody fragment consisting of a single monomeric variable antibody domain, and lacking the light chain and CH1 domain of the heavy chain. Like a whole antibody, it is able to bind selectively to a specific antigen. With a molecular weight of only 12–15 kDa, nanobody are much smaller than common antibodies (150–160 kDa), and even smaller than Fab fragments (~50 kDa, one light chain and half a heavy chain) and single-chain variable fragments (~25 kDa). The first nanobody was engineered from heavy-chain antibodies found in camelids; these are also called VHH fragments.
Increasing corporations and researchers’ seeking for nanobody service to enhance their industry gives rise to the development of nanobody service provider, and among them, there is Creative Biolabs.
Nonobody Service Provided by Creative Biolabs
Creative Biolabs is a recognized leader in nanobody production and single domain antibody library fields. They are specialized in generating nanobodies against any targets. They also provide a wide range of services, including construction of immunized single domain antibody libraries using llama and camel, construction of synthetic camelised human single domain antibody libraries using DNA synthesis, bio-panning of single domain antibodies libraries and large scale production of recombinant single domain antibodies. Together with Creative Biomart & Creative Diagnostics, they provide a large list of nanobodies.
Engineering and Manufacturing of Single Domain Antibody
Once the most potent clones are identified, their DNA sequence is codon-optimized and expressed in either bacteria cells or mammalian cells. Creative Biolabs is also able to engineer single domain antibodies in a wide range of formats, including unique multivalent, bivalent and bi-specific molecules. They are experienced in conducting affinity maturation of nanobodies. Quantitative library sorting by phage display or yeast surface display is used for improving the affinity of single-domain antibodies.
Creative Biolabs is a service provider dedicates to supporting customers with a series of the best designed and qualified Nanobody services, including nanobody library services, nanobody matration services and nanobosy production services.