Protocols for Mouse Monoclonal Antibody Production

Custom monoclonal antibody is produced from antigens in peptide or protein or even small molecules. It is also the part of Boston Molecules antibody production services. Different methodologies are used to create the antibody for different purposes. For production of mouse monoclonal antibody there are specific protocols.

Immunization and Spleen Fusion

Immunization and spleen fusion are used to create hybridoma cell lines. They are two phases in the process of producing mouse monoclonal antibody. It involves development, production, as well as purification of these monoclonal antibodies. Usually the suppliers provide protocols for 5, 10, or 15 mouse in strains. Reliable producers always make it a point to report in-process ELISA testing and they provide samples to the customer enabling them to evaluate the product and take an informed decision about their uses. The customer is thereby able to decide about the animal to be used for fusion and the cell lines to be cloned and expanded and delivered.

Purpose of Mouse Monoclonal Antibody

The basic purpose of using mouse monoclonal antibody is to optimize them and creation of efficient protocols that would immunize the mice. It would also be used for creating fusions, screening of hybridonas, as well as for delivering cell lines. These can later be used on other species of animals first and ultimately on human anatomy.

Reliable Provider of Antibody

Usually the reliable and reputable provider of mouse monoclonal antibody would guarantee titer. After the completion of the initially planned schedule of immunization they would provide boosters ensuring that they would reach a quantifiable limit at the end of the process. In addition; such provider can also ensure customization of the antibody produced befitting the requirement and budget of the customer. Customization is normally based on screening results and the testing conducted by the customers at their end. Above all; the qualitative service provider also offers full service integration combining different protocols into a full set of service covering the process of antigen production through antibody production as well as purification.

Stages in Monoclonal Antibody Production

Different stages are there in the process of mouse monoclonal antibody production. Antigen preparation is considered to be the phase 0 whereas the immunization protocol and the ELISA checking is the phase 1. Similarly the phase 2 involves the fusion protocol as well as the screening of positive supernatants. Phase 3 is sub-cloning and the last two phases; 4 and 5 are production and purification.

Only when all these phases are covered appropriately the mouse monoclonal antibody process can be most productive.

Author Bio:

The writer is an expert in the field of Business with focus on Mouse Monoclonal Antibody and Humanized Monoclonal Antibody.Article's Source:http://bostonmolecules.livejournal.com/3048.html