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Cell-Cycle Arrest And Apoptosis Is Caused By P53 Over Expression

Author: Zhang Qing
by Zhang Qing
Posted: Apr 10, 2014

It would be appealing to speculate that the mixture of a a mainstream treatment and a senescence induced therapy may cooperate to entirely remove cancer. p53, an important growth suppressor or guardian of the genomeis mutated, deleted or inactivated in a variety of cancer. Just about all people papillomavirus infected cancer cells include wild type p53. p53 is nonfunctional as HPVE6 proteins abrogates its function either by ubiquitin-dependent and separate degradation, by inhibition of acetylation or by repressing p53-dependent downstream molecular pathways.Imatinib Glivec

Though, E6 colleagues with p53 for its degradation;there are contradictory studies on the inhibition and activation of p53 trails by E6.Ectopic term of p53 in cancer cells lacking p53 or harboring mutant and/or abrogated wild-type p53,have contrasting effects on cell-fate. In p53 null cancer cells, cell-cycle arrest and apoptosis is caused by p53 over expression. However, in virus infected tissues harboring wild-type p53, over expression of p53 doesn't induce cell cycle arrest and apoptosis. Till date there are only three reports explaining the effects of p53 over expression in HPV-positive tissues and results obtained keep enough scope for question. Difference among these reports may be because of variations in adenoviral multiplicity of infection. Taken together, the role of p53 over-expression in HPV-positive tissues remains obscure. In HPV-positive tissue, E6 operates at different hierarchal degrees in p53 path.

It degrades p53, p21and Bax triggering disability in cell-cycle arrest/ making p53 activation and apoptosis more difficult.With new advancements in effective gene delivery systems and the outlook of gene therapy making a come -back it’s probably that p53 dependent therapy may become possible. We created a novel isogenic HeLa cells with Tet-On regulated p53 phrase, to review the role of p53 in E6-positive cells. Tet-On system reveals tight on is without pleiotropic effects.Moreover and /off rules, swiftly higher induction ranges are achievable and the inducer, doxycycline, is well-characterized. p53 over expression does not advertise cell cycle arrest and apoptosis in HeLa cells. We illustrate that protein phosphatase 2A controls p53 features and its p53 is activated by inhibition, causing cell cycle arrest/apoptosis in vitro and tumor growth inhibition in vivo.Interestingly, cyclin dependent kinase 5 regulates p53 phosphorylation essential for its activation. Taken together, we suggest that no-genotoxically over expressed p53 could be triggered by curbing its dephosphorylation in HPV-positive cervical cancer tissues.

This strategy may be of therapeutic value in p53 associated gene therapies. This study features its influence on cell-cycle arrest and apoptosis in HPV-positive HeLa cells and the activation of over expressed p53. Under stress conditions p53 is stabilized by acetylation and phosphorylation at serine/threonine/tyrosine and lysine residues respectively. The serine phosphorylation at residues 6,9,15,20,33,37,46,315 and 392 represents a crucial role dependant on the nature of anxiety thus producing /or apoptosis and cell-cycle arrest. Unlike anxiety situation whereby p53 induction promotes cell cycle arrest or apoptosis, this study demonstrates that p53 over expression in HPV-positive cells does not induce cell cycle arrest or apoptosis though; it's claimed to take action in different cancer cell types. ATP-competitive ALK inhibitor

The reason for this distinction could be self-consciousness of mobile systems necessary for performing critical post translational changes which are required for string unique promoter collection of the genes liable for the induction of cell-cycle arrest or apoptosis by HPV.Equilibrium between phosphorylation and dephosphorylation of a protein like p53 is vital for its normal functioning in the cells.

About the Author

Numerologist Warda is hooked on OG-L002 fishing, collecting. And lastly her encouragement comes from socializing along with her companions.

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Author: Zhang Qing

Zhang Qing

Member since: Oct 29, 2013
Published articles: 172

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