New Technology Enables The Cloning and Expression of Thousands of Genes

Recently, researchers from Johns Hopkins University, University of Trento, and Harvard Medical School have developed a new type of molecular biology technology called ‘LASSO Clone’ that can successfully separate thousands of samples simultaneously. The technology can successfully and effectively separate thousands of long DNA sequences simultaneously, which may be more powerful than any previous technology. The researchers said that this technology can accelerate the production of proteins, which can help researchers rapidly discover new disease therapies and biomarkers of various diseases. The related result of this study is published in the research report of the journal of Nature Biomedical Engineering.

In the article, the researchers described that this new technology can be cloned simultaneously in one reaction and express thousands of protein-encoded DNA sequences. Historically, researchers have only been able to clone and express a single gene at a time. Professor Ben Larman, one of the researchers, said that, ‘Our goal is to help scientists clone and express the entire set of proteins in any organism with simple and cost-saving method. So far, researchers have only been able to conduct relevant studies on a number of model animals such as Drosophila or Mice.’

The researchers described a novel DNA capture chain, the LASSO probe tool they mentioned was able to target long-term adaptable single-stranded oligonucleotides, collecting this type of LASSO probe can help researchers capture the desired DNA sequence. Each targeted gene sequence can contain up to several thousand DNA base pairs, which is the standard size of the gene protein coding sequence. The new technology developed by the researchers is an improvement of an ancient technique called molecular inversion probes (MIPs) that can capture about 200 base pairs of DNA.

In proof-of-concept studies, LASSO probes can be used to simultaneously capture more than 3,000 DNA fragments from the E. coli genome, and the researchers succeeded in capturing at least 75% of the gene targets. More importantly, the researchers also discovered that the captured sequences can help scientists analyze the function of genes encoded by genes, and nd how it transmits antibiotic resistance to other susceptible cells.

Finally, the researcher Larman said that they are very excited about the potential applications of LASSO cloning technology. Furthermore, the researchers hope to expand the technology to screen and express the number of proteins which will have involve some values and significance for new therapeutic biomolecules development for a variety of human diseases treatment.

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