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Application of Refrigerated high-speed centrifuge

Author: Chason Chan
by Chason Chan
Posted: Jul 24, 2018

Refrigerated high-speed centrifuge is suitable for the qualitative analysis of serum, plasma and urea in medical products, bloodstains, clinical trials and biochemical laboratories. Floor Standing Refrigerated High Speed Centrifuge separations technology is a new technology developed based on the state of particles in a practical centrifuge.The refrigeration unit of the Refrigerated high-speed centrifuge uses vapor compression refrigeration. Vapor compression refrigeration consists of compressors, condensers, expansion valves, evaporators and other components. Its role is to reduce the temperature of the centrifuge chamber.High-speed refrigerated centrifuge microcomputer control, DC brushless motor, is a special equipment for rapid separation of mixed liquids, can be widely used in scientific research genetic engineering genetics life science biochemical medical and other units, for laboratory tests, biochemical tests, separation of suspension, etc. jobs. The machine is easy to operate and has a fast cooling speed. It is suitable for experiments with low separation, high centrifugal requirements and low temperature.The interface movement method of the Refrigerated high-speed centrifuge can use single-sector cups. After the biological macromolecule sample solution is filled into the analysis cell using the Schlieren optical system, the biological macromolecules leave the gas and liquid meniscus in the pool under the action of the centrifugal force field. Face to determine its settling velocity. If the sample is a protein solution, it can be configured to a concentration of 1 mg/mL. Before adding to the analysis cell, the sample is dialyzed against the buffer in advance. The other balanced single-sector cup is filled with buffer. The Refrigerated high-speed centrifuge meniscus should be as high as the sample-filling cup. The choice of centrifugation speed should be based on the fact that the target protein molecule can completely settle to the bottom of the tank in 1.5 to 2 hours.

Judging from the current situation of the use of Refrigerated high-speed centrifuges in our experimental center, the use of two large-scale instruments and equipment is relatively high. Preparation of animal serums, isolation of soy protein, concentration of animal viruses (Newcastle disease virus, bursal disease virus), and utilization of sucrose density Gradient separation and purification of EV71 virus, centrifugal nanomaterials, etc., involving animal medicine, food processing, biochemistry, life sciences and other disciplines, give full play to the efficiency of the use of centrifuges, to meet the experimental needs of related disciplines.

The commonly used Refrigerated high-speed centrifuge tubes used in laboratories are plastic and glass materials, plastics are more common, and the centrifuge tubes of glass materials are fragile and cannot be used in high-speed or Refrigerated high-speed centrifuges. The plastic centrifuge tube is made of polypropylene, polycarbonate, polyethylene, etc. The plastic centrifuge tube is transparent or translucent. The Refrigerated high-speed centrifuge can intuitively see the centrifugal condition of the sample, but it is easily deformed and corrosive against organic solvents. Poor, so the service life is shorter.

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Author: Chason Chan

Chason Chan

Member since: Apr 10, 2018
Published articles: 11

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