Everything You Should Know About PCR & Real-Time PCR Reagents

The polymerase chain reaction also known as PCR is a process for amplifying very tiny amounts of DNA so they can be visualized and assessed or used in further scientific processes. PCR is widely used in almost all branches of biology including molecular biology, microbiology, genetics, environmental science, food science, biotechnology, forensic science, and clinical diagnostics.

The PCR technique involves using an enzyme called DNA polymerase to amplify a piece of DNA. The original molecule of DNA is duplicated by the DNA polymerase enzyme, thus doubling the number of DNA molecules. Then each of these molecules is duplicated in a second cycle of replication, resulting in four molecules. Again, each of these molecules is duplicated by the enzyme in a third cycle of replication. The process is referred to as a chain reaction - hence the name "polymerase chain reaction". The original single piece of DNA is amplified over many cycles, generating millions of copies of the original DNA molecule.

Often the PCR experiment is performed simply to verify the presence or absence of a particular type of DNA but sometimes PCR is used to generate sufficient DNA to be used in a subsequent experiment, for example gene cloning services. The original PCR process has been extensively modified to perform a wide array of genetic manipulations, diagnostic tests, and for many other uses. Modern variations of PCR include Real-time PCR. This technology allows the newly generated DNA molecules to be analyzed immediately they are produced. This ability to monitor the PCR process in real time, while the reaction is still occurring, is of enormous benefit to scientists as it reduces the amount of time required to generate a result (especially important in clinical diagnostics) and also enables the quantitation of the DNA. Real-time PCR can not only answer the question "what DNA is present?" but also "how much DNA is present?".

Molecular Biology research requires the use of high purity Real-Time PCR reagents and chemicals to ensure accurate results. Real-Time PCR Reagents may include several types of Taq polymerase, dNTPs, buffers, kits, and Master Mixes, which are premixed, ready-to-use solutions. Whether the technique involved is routine PCR requiring yes/no sequence detection, RT-PCR, hot start PCR, Q-PCR, long PCR, or site-directed mutagenesis, users are looking for Real-Time PCR reagent consistency, speed of amplification, precise DNA copying, and specificity to the target DNA sequence.

There are many online dealers that let you buy Real-Time PCR reagents and media kits reagents. These Real-Time PCR reagents can simply provide you with instant access to high throughput PCR automation, offering cost-effective solution and satisfactory results.

I love writing a lot and currently working as a Content and Academic Writer. My writing is qualitative, professional and timely which my clients like about me.