Overview on Acetyl-proteomics Analysis Platform

Acetylation is a highly conserved, reversible protein modification in vivo that plays an important role in the activation of transcriptional regulators in the nucleus. In addition, a large number of non-histone acetylation modifications are involved in the regulation of metabolic pathways and metabolic enzyme activities. It mainly takes part in regulating gene expression through modifying nuclear histones, but can also regulate several metabolic enzymes and metabolism pathways. There has been over thousands of acetylated proteins identified in proteomics studies. However, most acetylated proteins has very low abundance, hence, enrichment of acetyl-peptides can help to increase the sensitivity of mass spectrometry analysis.

MtoZ Biolabs, a biotech-company specialized in quantitative proteomics and metabolomics services, developed acetyl-proteomics analysis platform based on Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos mass spectrometry platform and Nano-LC.

Due to the low content and wide dynamic range of acetylated proteins in biological samples, the acetylated peptides need to be enriched to improve their abundance before mass spectrometry, and then the enriched acetylated groups are obtained by traditional quantitative proteomics analysis. The peptide samples are subjected to quantitative analysis. MtoZ Biolabs uses peptide pre-separation to reduce the effect of high-abundance histones on protein acetylation identification, and then combines immunoprecipitation with high-efficiency antibody-enriched acetylated peptides to identify and quantify large-scale acetylation. The selected kit is an internationally accepted acetylated peptide-specific enrichment kit supplied by CST. In order to identify and quantify more acetylated peptides, they use 2-3 different enzymes to digest the protein samples during the digestion process, ensuring that the peptides with acetylation sites can entered into the mass spectrometer for analysis with the way of high-efficiency ionization to eliminate the loss of acetylation information due to the inappropriate peptide length and low ionization efficiency.

Acetylation is a highly conserved, reversible protein modification in vivo that plays an important role in the activation of transcriptional regulators in the nucleus. In addition, a large number of non-histone acetylation modifications are involved in the regulation of metabolic pathways and metabolic enzyme activities.

Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos mass spectrometry platform and Nano-LC are used by MtoZ Biolabs to launch the acetylated quantitative proteome analysis service. Researchers only need to tell them the purpose of their experiment, and send out the samples. The company will be responsible for all subsequent matters of the project, including protein extraction, protease digestion, acetylation peptide enrichment, peptide separation, mass spectrometry, mass spectrometry analysis, and bioinformatics analysis.

Key Features

• High-through analysis, and quantitatively analyze several sample at once, when coupled with sample labeling
• Large scale analysis of acetyl-proteins, including low abundance proteins and peptides
• Highly accurate Nano LC-MS/MS detection system
• Professional MS data and bioinformatics analysis

To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

Reports

• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Peptide identifications and intensities
• Protein identifications and acetyl-site mapping
• Bioinformatics analysis

Prime Jones is a senior researcher from MtoZ Biolabs. She is specialized in the field of proteomics study.