Application of Membrane Protein Expression in E. coli—A Neo Hope to Recombinant Protein

Overview

Escherichia coli (E.coli), a kind of gram-negative Brevibacterium recognized as an opportunistic pathogen for human and livestock, which under certain circumstances may cause meningitis, diarrhea, septicemia, mastitis in cows and dairy goats. In spite of its pathogenicity, the E.coli is nowadays a crucial tool for recombinant protein construction, which is a kind of vaccine carrier, effectively expressing foreign antigens and bringing prospects to the biomedical therapeutics like vaccine development, with the following merits.

l Unparalleled fast growth kinetics

l High cell density cultures

l Cheap complex media

l Fast and easy transformation with exogenous DNA

The emergence of engineered proteins has greatly expanded the types of proteins that can be adopted in biochemical and structural biology researches. It’s worth noticing that in about 3 years at the start of the 21st century, 31 kinds of recombinant proteins as therapeutic proteins were approved by FDA. Many biotechnology companies arouse at the historic moment, among which Creative Biolabs is the leading one providing comprehensive one-stop solutions for recombinant protein and antibody development. Noting that the worlds’ majority of medical institutions and researchers are working on the recombinant protein to make varied the existing therapies, Creative Biolabs has developed technical membrane protein expression processes on different cells entitled Magic.

l Membrane Protein Expression in E. coli

l Membrane Protein Expression in Yeast Cells

l Membrane Protein Expression in Baculovirus-Insect Cells

l Membrane Protein Expression in Mammalian Cells

l Membrane Protein Expression in Photosynthetic Bacteria

The systematic workflow of Magic Membrane Protein Expression in E. coli composes expression, purification, stabilization, and characterization based upon the first-level cell system, which is embedded with the high-end technology platforms and experienced scientific staff to make sure satisfactory deliveries. Characterized and cGMP certified techniques involved in are BacTEC and FoldEZ, respectively enhancing the expression level and refolding and dissolving the inclusion bodies more than 98%. With the tools, all the E. coli cells will go through speedy loading and end with high yield, in a cost-effective manner. Aside from the Escherichia coli cells, other bacteria categories are also accepted as the hosts of membrane protein production, like Lactococcus lactis (L. lactis), Bacillus subtilis (B. subtilis), and Rodhobacter sphaeroides (R. sphaeroides).

At present, many schemes are utilized to achieve the soluble expression of recombinant protein in the cytoplasm or periplasmic space of E.coli, which have varied effects due to different protein types and various methods applied. Creative Biolabs has developed efficient approaches of bacteria destruction, purification, and is still exploring to optimize the yield of soluble recombinant protein. For years, researchers have made efforts to develop new therapeutic methods like recombinant protein to improve human health. It’s also the faith of Creative Biolabs to help the researchers to relieve the back issues of economic shortage and lack of techniques.

About the Company

Creative Biolabs, founded by a group of experts, is the leading custom service provider possessing extensive experience with over 10 years history in the field of various antibody production and engineering with service portfolio that includes mouse and rat monoclonal antibody production using hybridoma technology, human, monkey, rabbit, chicken, dog, llama and camel monoclonal antibody production using various antibody library technologies (including phage display, bacterial display and yeast display).

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