Facts and Figures About Prolactin ELISA Kit

Background of Prolactin

There has no cross-reaction with human LH, FSH, hGH, and hCG with Prolactin ELISA kit. In men and women both, the Prolactin hormone is hidden from the pituitary glands. The molecular weight of the prolactin is 23,000 approx. with a single chain polypeptide hormone. The prolactin is synthesized and released under neuroendocrinal control. The basal prolactin level is high in women as compared to the basal prolactin level in men.

Level of Prolactin

Estrogen rise apparently and menopause decreases. Human prolactin initially used to maintain lactation and in breast development. The gonadal function also decreases with the prolactin. Prolactin level progressively increases to 10 to 20% in pregnancy and start declining in 3 to 4 weeks of postpartum. Prolactin goes to a high level in breastfeeding mothers; serum concentration takes months in returning non-pregnant levels. The hypothalamic-pituitary disorder can be identified with the help of prolactin concentration.

Hyperprolactinemia related to male impotence may be caused by Microadenomas. Amenorrhea and galactorrhea are related to high Prolactin. Thyrotropin-releasing hormone, estrogens, and dopaminergic mechanisms are used to increase the prolactin in humans.

In different situations like stress, hypoglycemia, and exercise, the level of prolactin is assessed by hypothyroidism and renal disease. It releases in demonstrates variations and in an episodic manner. The concentration of prolactin can be increased by several drugs like reserpine, chlorpromazine, and may be decreased by L-dopa and bromocriptine.

Facts and figures about Prolactin ELISA kit

Prolactin is the pituitary hormone and is used to stimulate the regulation in water and salt, reproduction, growth, milk production, etc. with a pathway, PRL puts the impact on different types of cells. The binding of hormones goes to the PRL receptor transmembrane.

Prolactin ELISA uses a technique known as a quantitative sandwich immunoassay. The kit includes the microtiter plate. The monoclonal antibody is used to coat these microtiter plates. This monoclonal antibody is used specifically for prolactin. Then, microtiter plate wells are added to the samples or standards. The prolactin is than bind to the antibodies that are coated with the wells.

The incubation process is done on the microtiter plate and then to remove the unbound parts, it washed thoroughly. On the next step, a substrate solution called TMB is added in every well.

In a very short period of incubation, the substrate and HRP enzyme reacts.

Wells, having enzyme-conjugated antibodies and prolactin reacts and shows the change in colour. The solution of sulphuric acid is added to the enzyme-substrate, the reaction stops immediately. With Wavelength of 450 nm of spectrophotometrically measure colour change, soon after the reaction terminates.

In the samples, prolactin concentration is measured by O.D samples with the standard curve.

Content of Prolactin kit

• 96 coated well plates
• Standards
• Wash buffer
• Chromogen
• Stop solution
• Assay diluent concentrate
• Plate covers
• Conjugated detection antibody

The Prolactin kits are tested by the manufacturer against precision accuracy, sensitivity, specificity, and many more factors. These are used in different research laboratories for the purpose of research.

Storage of kit

Kit could be stored for the whole year, soon after shipment, if you place it on -20? C. on 4? C it could be stored for 6 months. At -80? C it can be maintained for an extra-long period of time.

Digital marketing professional with expertise in developing an Seo strategy. I have Years Of Experience In Seo & Digital Marketing. As an Seo Specialist.