Application of PEG In Click Chemistry

Polyethylene glycol (PEG) is a biocompatible, synthetic, hydrophilic polyether compound that has many applications, mostly in the medical industry, but also in the chemical and industrial sectors.

The synthesis of PEG is done by polymerizing ethylene oxide, the main ingredient in antifreeze, using a ring-opening technique, which allows for PEGs of a range of molecular weights and molecular weight distributions to be constructed. This range in weights is what makes it suitable for several uses.

Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules.

One of the earliest and most important methods in bioconjugation was to express a reporter on the same open reading frame as a biomolecule of interest. Notably, GFP was first (and still is) expressed in this way at the N- or C- terminus of many proteins. However, this approach comes with several difficulties. For instance, GFP is a very large unit and can often affect the folding of the protein of interest. Moreover, by being expressed at either terminus, the GFP adduct can also affect the targeting and expression of the desired protein. Finally, using this method, GFP can only be attached to proteins, and not post-translationally, leaving other important biomolecular classes (nucleic acids, lipids, carbohydrates, etc.) out of reach.

Click Chemistry has been widely used in bioconjugation, biolabeling and material sciences in pharmaceutical and biotech industry. It refers to a group of reactions that are fast, simple to use, easy to purify, versatile, regiospecific, and give high product yields.

Biochempeg is especially good at click chemistry reagents which now widely used on biomolecular probes, two-dimensional gel electrophoresis separation, modification of peptide function with triazoles and natural products, and pharmaceuticals, nanotechnology, Biomaterials, etc.

Biochempeg provides high purity Click Chemistry tools with a broad range of functional groups: Azide, Alkyne, DBCO, BCN, etc. Activated PEG products with molecular weights, branching and functional groups not listed in our website may be available by custom synthesis.

PEG Reagents for Click Chemistry

Azide Reagents

Azide Reagents enables Click Chemistry and can be used to react with amine-containing biomolecules, modifying carboxylic acid groups, and etc.

ex. N3-PEG-Mal

Alkyne Reagents

Alkyne reagents can be reacted with azide-bearing compounds or biomolecules via CuAAC reaction to yield a stable triazole linkage.

ex. Alkyne-PEG-IA

DBCO Reagents

DBCO (Dibenzocyclooctyne) reagents is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (SPAAC), which enables Cu-free Click Chemistry.

ex. DBCO-PEG-NHS

BCN Reagents

BCN reagents (bicyclo[6.1.0]nonyne) can react with azide-tagged molecules or biomolecules via copper-free Click Chemistry.

ex. endo-BCN-PEG2-OH

Biochempeg is a biotechnology-oriented company in Watertown, Massachusetts. We are dedicated to manufacturing and supplying high purity polyethylene glycol (PEG) derivatives and reagents, monodisperse PEG, Click Chemistry reagents, PEGylation services and custom PEG derivative synthesis to clients worldwide. These PEG reagents have been widely used in bioconjugation, antibody-drug conjugates (ADCs) therapeutic, drug delivery and diagnostics field.

I am working in Hunan Huateng Pharmaceutical Co., Ltd.