Tunable SERS-Tags -- a Hidden Gold Nanorattles for Theranosis of Cancer Cells with Single Laser Beam

Research from AIA Research Group shows that Increased N-glycosylation of Asn88 in serum pancreatic ribonuclease 1 is a novel diagnostic marker for pancreatic cancer.

The study shows that N-glycosylation at Asn88 in serum RNase1 was significantly increased in patients with PaCa. This finding was established using an immunoassay that was developed to determine the level of total RNase1 compared with that of RNase1 that was not N-glycosylated on Asn88 (Asn88-free). The increase levels of RNase1 with N-glycosylated Asn88 in the sera of patients suspected of having PaCa thus shows promise as a novel diagnostic marker for screening and detection of PaCa. The immunoassay and Western blot-base analysis were developed for detecting this change in N-glycosylation of RNase1 using RrhRN0723 mAb and RN3F34 mAb, respectively. Both antibodies directed against human RNase1 recognize the peptide region surrounding the Asn88 N-glycosylation site containing unglycosylated Asn88, but not when its Asn88 is N-glycosylated. The EIA analysis using RrhRN0723 which allows the high-throughput and quantitative detection of the levels of Asn88-free RNase1 is suitable for clinical purpose. In contrast, the Western blot analysis using RN3F34 which allows the qualitative detection of the changes in N-glycosylation at Asn88 is good for research tool rather than clinical purpose.

Research from Zhaolong Chen, Dexin Yu, shows that Tunable SERS-Tags-Hidden Gold Nanorattles for Theranosis of Cancer Cells with Single Laser Beam.

In this study, STHGNRs for theranosis of cancer cells were produced by a galvanic replacement reaction between GMSSNs and HAuCl4. Their observed red-shift of SPR peak with increase of HAuCl4 is mainly due to reduction of the shell thickness (figure 1), which is consistent with our theoretical analysis. On one hand, increasing void size while fixing the inner core size and shell thickness constant, will contribute to the absorption band red-shift as the plasmon oscillation decreases in energy. This result is consist with the optical properties of hollow gold nanospheres. On the other hand, increasing shell thickness while fixing the inner core size and void size constant, will contribute to the SPR peak blue-shift. This result implies that STHGNRs takes on more solid-particle-like properties as inner cavity decreases and shell thickness increases. The SPR peak of 13 nm GNPs is approximately 520 nm. When it was trapped in hollow gold shell, the SPR peak would shift to lower energy, which can be explained with Halas' Plasmon Hybridization Theory. The SPR peak of STHGNRs was readily tuned to NIR required for PTT and SERS detection by controlling the inner void sizes and shell thicknesses.

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