Methyltetrazine-Amine – Important of Tetrazine in Click Chemistry

Click chemistry is not a new field of study, though it is not very well known to all. It is basically an advanced level bio-chemistry through which DNA structures are simplified to be analyzed. With the advent in technology, bio-chemistry has noted some massive changes. Not just only origins of some complicated diseases have been recognized, but also a lot of advanced level life saving medicines have been produced with the help of bio-chemistry. When it comes to click chemistry, reagents are considered to be part and parcel of the process. Reagents are basically used to detach the amino group from the DNA structures so that the structures can be made simplified to be read.

A Guide to Methyltetrazine-Amine

Amine functionalized tetrazine has been often used for inverse demands of electron Diels-Alder cycloaddition reactions. The tetrazine is known to be a substance that reacts with alkenes such as norbornene, transcyclooctene and cyclopropene. The motto of this reaction is to obtain a covalent as well as stable linkage. In case of bioorthogonal reactions, Tetrazines has proven its usefulness for different kinds of biological imaging and bio-conjugal applications. Methyltetrazine-Amine is an advanced level derivative of tetrazine.

Methytetrazine-PEG4-Amine has a major role to play in click chemistry. For protein and protein bi-conjugation, this reagent has been considered as a powerful tool in click chemistry, enabling the operating power without the need of any catalysts at a given temperature level and pressure condition. Tetrazine can be of different types and various types of tetrazines are also considered as reagents. For example, Methyltetrazine-PEG4-Maleimide is another strong reagent that has been used for protein conjugation, sampling and labeling. In the next phase of this article, we shall discuss about different properties of tetrazines.

Properties of Methyltetrazine-Amine

Here are some of the properties of Methytetrazine-amine at a glance for you:

• Bio-compatible: Tetrazine is highly bio-compatible and it can work under mild buffer conditions. No copper catalyst is required for this reagent, under normal temperature as well as pressure conditions. This is why it is considered to be one of the most costly reagents.
• Chemo-Selective: Tetrazine is highly chemo-selective in nature. Reagents, like Methyltetrazine-PEG4-Ester or others, do not react with functional groups that are often found in the biological samples. However, for conjugation process, Methyltetrazine has been used.
• Solubility: Methyltetrazine is soluble in water and the aqueous solution has been used for performing reactions.

Methytetrazine-PEG4-Acid and other Methytetrazine reagents are available in the marketplace though most of them are highly costly.

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