General Introduction to Nanobody

1.Basic Information of Nanobodies

Nanobodies are a unique form of monoclonal antibodies, characterized by a single antigen binding domain, present in camels. With a molecular weight of only 12–15 kDa, nanobody are much smaller than common antibodies (150–160 kDa), and even smaller than Fab fragments (~50 kDa, one light chain and half a heavy chain) and single-chain variable fragments (~25 kDa).

The Department of Cellular and Molecular Interactions discovered the unique properties of camelid antibodies several years ago and subsequently optimized the nanobody technology and its applications. The first nanobody were engineered from heavy-chain antibodies found in camelids; these are also called VHH fragments.

The size reduction of an antibody into a nanobody (and the concomitant reduction in valency from bivalent to monovalent) can cause a dramatic change in biological activity, which provides many advantages over conventional antibodies and their recombinant fragments.

Firstly, like conventional antibodies, Nanobodies show h Nanobody

igh target specificity, high affinity for their target and low inherent toxicity. Secondly, Nanobodies are generally very stable and have the potential to be administered by means other than injection thus are easy to clone, therefore they are to be expressed with high yield.

However, like small molecule drugs they have the opportunity to inhibit enzymes and readily access receptor clefts, which is challenging with conventional antibodies.

Nanobodies have a wide range of applications and can be used in immunohistochemistry, immunomodulation, intracellular expression (intrabodies), biosensor-applications and so on.

2. Nanobody Service of Creative Biolabs

Creative Biolabs was founded by scientists who are dedicated to the conquering of cancer, and we are the leading custom service provider that has extensive experience in various antibody production and engineering fields.

Besides, Creative Biolabs is the recognized leader in nanobody production and single domain antibody library fields. We are specialized in generating nanobodies against any targets. We can provide you a wide range of services, including construction of immunized single domain antibody libraries using llama and camel, construction of synthetic camelised human single domain antibody libraries using DNA synthesis, bio-panning of single domain antibodies libraries and large scale production of recombinant single domain antibodies. we provide a large list of nanobodies.

In terms of Nanobody Service, we can provide you Single Domain Antibody Library, based

on phage display technology; Single domain antibodies (Nanobody) Affinity Maturation Services, with affinity maturation of single domain antibodies in particular;Human or Humanized Nanobody Production Services, using humanized single domain antibody scaffolds. We would appreciate to cooperate with you.

Creative Biolabs is a recognized service provider in converting small gene-engineered scFv or Fab human or mouse antibodies derived from phage display antibody library screening.