What are the Factors to be Considered During Gas Chromatography

The article describes the features to be considered when a gas chromatography experiment or testing is done, and these important points have to be kept in mind.

The main challenge that you face during performing gas chromatography India are control on the factors which influence the separation of the components. Hence a close understanding of the factors helps you control the chromatography process better, and get to valuable conclusions of substances present.

The Factor of Vapor Pressure

The polarity of a compound determines its boiling point. In fact the vapor pressure and boiling points are inversely proportional. The lower the boiling point is the higher the vapor pressure and vice versa. And with higher vapor pressure the retention time of the compound gets shorter. This means the compound will be staying lesser time in the gaseous phase. Exactly for these reasons, solvents with low boiling points are used, and the solvent has to dissolve the solute or sample that is to be tested. To get the vapor it’s not essential that the temperature be higher than the boiling point, rather some vapor is emitted in every compound even at non-zero vapor pressure which makes you detect many compounds by their smell only.

Polarity of Stationary Phase versus Components on Column

The retention time gets enhanced if the polarity of the stationary phase and the compound matches. This happens because the interaction of the compound with the stationary phase gets stronger. Hence, the retention times of the polar compounds are longer on the stationary phases. And in the non-polar columns the retention rimes are shorter, while temperature is the same in both cases. Cyclodextrins, amino acid derivatives and the chiral silanes, which determines the stationary temperatures, has the capability to separate enantiomers. That is because, the interaction of the enantiomers are stronger than the other stationary phase compounds.

Flow Rate of Carrier Gas

The retention time is reduced due to a high flow rate, and also a rate of poor separation is noticed. The time of the components to interact is little through the stationary phase, and by the column they get pushed.

The Impact of Column Length

The separation of the column is improved when the column length is long. As the column length increases, the retention time also increases proportionally, and because of the longitudinal diffusion which is increment in the column, a peak broadening is noticed. And here it’s much important to note that the motions of the gas molecules are in all sides and ways. The flow rate and the broadening are again inversely proportional.

Injected Material Amount

Normally the ideal chromatography peaks are in a Gaussian curve. Now the peaks changes shape and shows tailing that is significant when too much content gets injected, and this makes the separation poor. Detectors are mostly sensitive, and don’t require a huge amount of the material for showing results or signals.Practically only 1-2 percent of the compound that is injected can pass through the injection port and to the column, because almost all GC instruments are protected from overloading through the split mode operation.

The main factors in gas and liquid chromatography India are discussed herein, and these contribute to the process and decide how accurate the results would be.

Important points about the gas chromatography India reaction are discussed so that errors in the process can be avoided, and conflicts can be totally avoided. The more one understand the process and influential factors about liquid chromatography India it will be easier to continue the experiments.

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