Uncovering The Popularity Of Rna Seq Analysis

RNA sequencing has transformed the manner in which the transcriptome is studied. This method has permitted researchers to accurately measure changes in expression across the transcriptome. In other words, researchers now have the ability to clearly analyze and detect changes in tissues/cells that were hitherto undetected. Consequently, it is now possible to fully understand the changes that occur as a response to intervention. These insights are possible across every conceivable environmental condition, offering an in depth and accurate analysis of the changes.

One of the advantages of RNA Seq Analysis is the ability to unravel features that are both identified and unidentified. As a result of this ability, researchers can now detect gene fusions, variants and multiple other markers/features without being constrained by the need for prior information about the new features.

Enhanced accuracy and sensitivity

There is a near unanimous preference among researchers studying the transcriptome for Illumina RNA Seq in combination with NGS. This is primarily because this offers greater accuracy in measurement of gene expression with improved sensitivity. Additionally, it permits research without the need for being aware of the new features. Similarly, the ability to apply it to all species in the absence of reference material makes it a great research method. Other reasons for the popularity include the very important criteria of cost; this offers better value for the expenses in terms of price per sample. A process that used to typically take months and possibly years to conclude is now completed in a few hours or days. The combination of accuracy, speed and costs makes this the best choice for research.

Dynamic range and detection of unknown features

Accurate identification of (DEGs) differentially expressed genes is pivotal to understand variations in phenotype and RNA Seq, by virtue of being high throughput transcriptome sequencing has emerged as advantageous. The technical reasons that make this advantageous are manifold – wider dynamic range, improved sensitivity, greater accuracy, detection of known and unidentified features, ideal method for model and non-model species.

Culture free method of 16S rRNA Sequencing

Similarly, the use of 16S rRNA Sequencing in diagnostic laboratories has remained popular primarily due to the twin facts that the function of the 16S rRNA gene has remained unchanged over time and the gene is very large, offering better opportunities for bioinformatics and analysis. The culture free method used in the identification of strains from complex environments that are not possible with other traditional methods, makes this the best method to identify and conduct comparisons of bacteria in samples. The use of 16S rRNA Gene Sequencing mandates the need for laid down set of guidelines that will help interpret data in a manner to offer accurate comparison of results among studies. There have been numerous recommendations regarding the need for criteria for full 16S rRNA gene sequencing. This permits accurate identification of species and will be of immense use in a diagnostic setting. The adoption of advanced technology and standards as a corollary to the previous DNA-DNA hybridization will help improve standards. It is necessary to adopt standards early, as this will help to enhance developments, resulting in evolved standards that meet the dynamic requirements.

Author Bio:

John Taylor is the Senior Marketing Manager of 1010Genome Pte Ltd. 1010Genome is a next generation sequencing and bioinformatics data analysis service provider. Our portfolio of services includes Whole genome, Exome, RNA-seq, transcriptomics, gene mapping, cancer genomics and metagenomics sequencing and data analysis.